C-ID Descriptor
Applied Biotechnology with Laboratory

None

Algebra (C-ID MATH 150 or C-ID MATH 151), English (C-ID ENGL 100), Biology course that includes a lab, Introductory Chemistry (CHEM 101)

Lecture Topics:

Scientific method

Laboratory math

• Units of Measurement and Converting between Units (Metric System)
• Graphical Methods of Analysis and Data Display
• Various Methods of Expressing Concentration
• Calculation for Molar and Percent Solution
• Dilutions
• Reagents, Solutions and Media Formulations

Basic chemistry of buffers and pH

Biotechnology overview

Work-readiness skills

• Oral communication
• Written communication
• Teamwork

Health and Safety

• MSDS/SDS
• Personal Protection Equipment
• Contamination

Metrology: Common Laboratory Measurements

• Weight
• Volume
• pH
• Temperature
• Spectrophotometry

Quality Control

• Biological molecules
• Cell Structure
• Gene expression
• DNA technology and analysis
• Cell culture and fermentation
• Bioseparation (protein purification, agarose gel, etc.)

• Basic separation methods and analysis (at least 4 of the following)
  • DNA electrophoresis
  • Protein electrophoresis
  • Column chromatography
  • Protein assay (quantity)
  • Protein assay (function)
  • Centrifugation
  • Filtration
• Other Protocols (at least 4 of the following molecular techniques)
  • Plasmid isolation
  • PCR
  • Restriction digestion
  • Bioinformatics
  • Nucleic Acid Technology
  • Western Blot
  • ELISA
• Inoculation/growth of cell cultures
• Lab safety
• Metrology (pH, balance, volumes, micropipetting, spectrophotometry)
• Biological Solutions, including media preparation, autoclaving, pH determination and adjustment
• Aseptic technique
• Data collection and evaluation
• Documentation and record keeping in lab notebook

At the conclusion of this course, the student should be able to:

• Describe cell structure
• Apply principals of basic chemistry of buffers and pH to biological molecules
• Define and distinguish among biological molecules
• Construct the flow diagram of gene expression from DNA to protein
• Explain recombinant DNA
• Be able to convert between metric units
• Be able to perform basic dilutions
• Perform calculations related to reagents, solutions and media formulations
• Demonstrate Good Laboratory Practices and record keeping in a laboratory notebook
• Prepare and analyze graphs
• Grow cells using aseptic techniques
• Demonstrate ability to use measurement instrumentation properly
• Perform a concentration assay for DNA or Protein
• Successfully perform a basic bio-separation technique such as column chromatography
• Perform a molecular technique such as DNA sizing electrophoresis

A variety of assessment techniques that may include midterm exam, term paper, projects, homework problems, lab reports, lab practical, and lab projects.  There should be a scientific communication component (eg. written reports, presentations, discussions). Documentation including lab notebook record-keeping in a regulated environment should be included in the evaluation section.

Introduction to Biotechnology (3rd Edition) by William J. Thieman and Michael A. Palladino (Jan 23, 2012)  ISBN 978-0321766113

Basic Laboratory Calculations for Biotechnology by Lisa A. Seidman (Apr 28, 2007)  ISBN  978-0132238106

Basic Laboratory Methods for Biotechnology (2nd Edition) by Lisa A. Seidman and Cynthia J. Moore (Nov 8, 2008) ISBN 978-0321570147

Laboratory Manual for Biotechnology and Laboratory Science: The Basics by Lisa A. Seidman, Mary Ellen Kraus, Diana Brandner and Jeanette Mowery (Nov 6, 2010) ISBN 978-0321644022

Campbell Essential Biology (5th Edition) by Eric J. Simon, Jean L. Dickey and Jane B. Reece (Feb 19, 2012) ISBN 978-0321772596

Biology by Sylvia Mader and Michael Windelspecht (Jan 3, 2012)  ISBN 978-0073525501

Basic Methods for the Biochemical Lab (Springer Lab Manuals) by Martin Holtzhauer (Jul 19, 2006)

ISBN 978-3540327851

Biotechnology: Academic Cell Update Edition by David P. Clark BA (honors) Christ's College Cambridge 1973, PhD University of Brsitol (England) 1977 and Nanette J. Pazdernik (Jan 12, 2011) ISBN 978-0123850638

Biotechnology: Science for the New Millennium by Ellyn Daugherty (2007) ISBN 978-0008453138

Principles of Biology by Nature Education (2011: online, “living” text) Nature Publishing group

Molecular Biotechnology: Principles and Applications of Recombinant DNA by Bernard R. Glick and Jack J. Pasternak (Nov 1, 2009) 978-1555814984

Fundamental Laboratory Approaches for Biochemistry and Biotechnology by Alexander J. Ninfa and David P. Ballou (May 26, 2009) 978-0470087664

Biotechnology: A Laboratory Skills Course by Bio-Rad 978-0-9832396-0-4

Biomanufacturing Laboratory Manual.  Northeast Biomanufacturing Center and Collaborative.  2012.